454 pyrosequencing v1–v3 regions,16srrna  (Pyrosequencing Inc)

Journal: Journal of Dental Sciences

Article Title: Oral microbial profiles of extrinsic black tooth stain in primary dentition: A literature review

doi: 10.1016/j.jds.2024.02.028

Figure Lengend Snippet: Oral microbial profiles of the EBS in primary dentition.

Article Snippet: 2016 , China , 40 EBS; 26 HG , saliva plaque EBS , 454 pyrosequencing (V1–V3 regions,16SrRNA) , Relative abundance: • Plaque-EG vs. HG: Leptotrichia and Fusobacterium ( P < 0.05–0.001, EG↑) • Saliva-No significant difference.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Molecular Accounting and Profiling of Human Respiratory Microbial Communities: Toward Precision Medicine by Targeting the Respiratory Microbiome for Disease Diagnosis and Treatment

doi: 10.3390/ijms24044086

Figure Lengend Snippet: Summary of the published studies related to the respiratory microbiome in COPD patients.

Article Snippet: 2011 , Healthy smokers, nonsmokers, and COPD subjects, USA. , 4 , To explore the differences in the lung microbiome of the three groups. , 16S rRNA pyrosequencing (v1-V3 region). Taxonomic and phylogenetic-based analysis l6s rDNA. The qPCR was used to quantify the total bacterial load. , Healthy smokers, nonsmokers, and mild COPD tend to have a more diverse microbial community than moderate and severe COPD microbiota. There was no significant difference in the microbiota and the bacterial load between the three study groups. A core microbiota was identified and includes: “ Pseudomonas , Streptococcus , Prevotella , Fusobacterium , Haemophillus , Veillonella ”. Significant microanatomic changes in bacterial population were observed within the same lung of advanced COPD patients. , [ ] .

Techniques: Microarray, Biomarker Discovery, Control, Bacteria, Sequencing, Sterility, Positive Control, Cloning

Journal: BMC Nephrology

Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature

doi: 10.1186/s12882-020-01805-w

Figure Lengend Snippet: Characteristics of included studies

Article Snippet: Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported.

Techniques: Medications, Sequencing, Control, Amplification, Real-time Polymerase Chain Reaction, Generated, Microarray, Sterility, Sampling, Transplantation Assay

Journal: Pediatric research

Article Title: Bacterial microbiome of breast milk and child saliva from low-income Mexican-American women and children

doi: 10.1038/pr.2016.9

Figure Lengend Snippet: Summary of comparison populations.

Article Snippet: Cabrera-Rubio et al. , Caucasian women in Finland , Mothers , 18 , 100% , Breast milk, self-collected near delivery , 32.0±5.1 yr , Barcoded 454-pyrosequencing of V1–V3 region.

Techniques: Comparison, Illumina Sequencing, Sterility, Transferring